FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_50220371-2-mss.3510000003eff2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_50220371-2-mss.3510000003eff2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219414
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA84700.016.8656331
GTATATA90900.016.6555121
TACACTG81800.016.4171585
GTACTAG24900.016.1813531
GTATACG6807.819246E-616.1597181
TATATAG54650.016.0842535
CCTATAC34000.015.0812483
GTATTAC35950.014.9435561
GTATTAA56350.014.5171391
GTATTAT58100.014.5001721
GTATAGG33700.014.4920651
TATACTG55800.014.4400385
GTATAGA45200.014.31652451
CCACGAA28600.014.079159245
TCTATAC48150.013.1848133
ATATAGT58350.013.1811746
TATAATA87400.013.1305622
GTGTAGA58750.013.092811
TAATACT61800.012.8407914
GTATAAG46750.012.7971911
Produced by FastQC (version 0.11.2)