FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_44065-mss.3510000003efbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_44065-mss.3510000003efbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544405
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG42750.018.8504521
GTATACG7854.64388E-918.6648921
GTGTATA70550.017.3068071
GTATAGG28750.016.5630651
ACGGACC16900.016.518969245
GTATTAA49050.016.4292931
TATACTG46900.015.6185
TATATGT94050.015.44702154
GTCCTAG35400.015.1762111
GTATAGA39200.014.9509621
TAGTGCG4900.00384649814.946529
TATACAG51800.014.8476525
GTATTAT48400.014.631761
GGATCGT17550.014.6058598
GGACCGA7552.1080677E-514.5526686
GCGTAGA6851.5017127E-414.2597971
GTATAGC23750.013.8807871
GTGTAGA47700.013.8225861
GTATATA78100.013.6013551
GTCCTAA37200.013.4572041
Produced by FastQC (version 0.11.2)