FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_35008-2-mss.3510000003ef95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_35008-2-mss.3510000003ef95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822293
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT33650.018.8674341
TCGTATT15700.018.779074245
CGCGACC5150.00107886318.078579245
TATACTG65950.017.2152425
GTCGTAA5002.234937E-417.093171
TCCTATA60650.016.908752
TAGCCCG5954.521569E-516.4141375
GCCCTAC26750.015.9749241
ACTCGCG6909.00442E-615.92118558
GTCCTAG36150.015.8739181
GTCCTAC25400.015.8626161
GTATAAG45050.015.4480851
CCTATAC35750.015.3673693
CTCCTAT64850.015.2499921
CTGTCGC21900.015.0487929
GTATTAG50150.014.6074661
GTATATA114400.014.514681
CCCTATA33650.014.5123572
TCTATAC53500.014.3763893
GCTAGAC43650.014.264253
Produced by FastQC (version 0.11.2)