FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_35008-1-mss.3510000003ef88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_35008-1-mss.3510000003ef88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267152
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG7800.026.6117211
CGCATGA2959.122214E-426.30122245
CCCCTAG6757.075869E-1021.7068141
GTCCTAT9900.020.9668081
CCTATAC9607.2759576E-1219.0752433
TCCTAGA17550.018.0861572
GCCCTAG7758.129609E-817.3304421
GTTACCC6959.61727E-615.8091213
CCCTATA9303.7684003E-815.7524592
GTATATA46200.015.5929641
CCTAGAG15750.015.5024193
GTGTAGC7901.8853661E-615.4558021
CCTAGAC11902.0736479E-1015.3884323
ATCCTAG13605.456968E-1215.2626041
CCCTAGG9605.5657438E-815.2601942
GTGCTAA12954.5474735E-1115.0858161
GTCCTAC6509.600717E-515.0277951
GTGTAAG13206.184564E-1114.80011
GTGTTAG14051.0913936E-1114.77376651
CTGGTAC10801.4775651E-814.6950023
Produced by FastQC (version 0.11.2)