FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_34310-1-mss.3510000003eef3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_34310-1-mss.3510000003eef3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43256130
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC218550.019.174837245
GTATATA556300.017.0750051
GTATAGG256950.016.1555081
GTGTATA508950.016.0007761
GTATTAG384450.015.9424181
TATACTG383750.015.9375265
GTATAAG354250.015.2336051
GTATAGA358950.015.1361831
GTATTAT425100.014.5040931
GTATTAA407900.014.3673891
GTGTAGG359950.013.97479251
GTATAGC253200.013.4533621
GTGTAAG414850.013.3026351
GTATAAT367400.013.2261741
TCTATAC368550.012.984833
GTCCTAG291500.012.8584951
TATACAG461100.012.8403885
TACACTG546250.012.4478785
ACTGATC502150.012.396778
GTATATG495850.012.3607191
Produced by FastQC (version 0.11.2)