FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_34302-1-mss.3510000003eee6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_34302-1-mss.3510000003eee6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578937
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG21950.019.4658411
GGATCGT11607.2759576E-1216.8363158
GTATATA71500.016.7324561
GTGTATA56300.016.4795321
ACTGATC53500.016.1990538
GTGCGCG4550.002345406716.0982821
ATACCGT8502.3391112E-715.7966466
GTGTAGG26550.015.6334231
TATACTG43000.015.6129645
AGGACGC8209.249248E-515.094914245
ATACTGT58650.014.98496156
GATCGTT14051.0913936E-1114.7692239
GTGTAAG41550.014.6905711
GTATATG48750.014.5242271
GTGTATG51700.014.4038621
GTATTAG38300.014.3434421
CCGTACC9407.3124284E-714.2844014
GCGTATA5250.006075626213.9518441
TCCGTAC10551.771914E-713.8843643
ACCGTTC7953.4335942E-513.8184858
Produced by FastQC (version 0.11.2)