FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_34298-1-mss.3510000003eecc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_34298-1-mss.3510000003eecc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050824
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG5307.226463E-720.7337151
GTATTAG32350.020.003781
GTATAGG21200.016.702161
GTATATA66700.015.9258981
GGATCGT10808.712959E-1015.823188
CCTATAC23150.015.8206313
TATACTG38600.015.8135415
TGTACCG4800.003349836515.2600685
TATACAG43950.014.7218865
GTATAGA31750.014.613371
GTGTAGA36100.014.5435941
CGTAGTA5050.004694521414.5048562
GTGTATA55950.014.4030461
GTATAAG32300.014.3645361
TACACTG44800.014.1700625
GTCTAGG29650.014.0011821
GTGTAAG36600.013.6777071
GTATAAT42000.013.6634091
ACTGATC42400.013.5307388
GATCGTT11754.491085E-813.50501259
Produced by FastQC (version 0.11.2)