FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_34274-2-mss.3510000003ee96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_34274-2-mss.3510000003ee96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020752
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC27650.018.984563
CGGACTA5200.001152221217.902061245
GTATATA103800.015.9976151
CCGTACC9354.035246E-815.6673984
CTCCGAC11103.1788477E-715.375344245
CCTGTAC39950.015.2784913
GTGTATA80350.015.0439881
GTCTAAG31450.014.7528741
CTCCTAT57700.014.1779241
GTCCTAA32000.014.1177471
CTAATAC50800.013.9377143
CGCTATG6200.001146531313.7826782
CCTACAC41000.013.6962363
CCCTTAG34850.013.6639261
GTCCTAG37900.013.5308091
GTATAAC28550.013.2577321
GTCCGGG6450.001542485713.2511031
GTGTAGG29850.013.0893881
ATCCTAG45850.012.7824941
GCTAGAC37300.012.763893
Produced by FastQC (version 0.11.2)