FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_34271-1-mss.3510000003ee7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_34271-1-mss.3510000003ee7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41267722
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA678350.016.5588041
GTGTATA598700.016.0086751
GTATAGG258350.015.4537631
GTATAGA399100.015.051451
TATACTG442650.014.3409825
GTATTAG416350.014.2225721
GTATAAT451950.013.7236071
GTATAAG388050.013.4034581
GTATTAT511350.012.8934891
GTATTAA478200.012.6894211
ACTGATC555600.012.6105388
TATATAC716650.012.4184843
GTGTAAG443300.012.0909871
TACACTG554800.011.9921235
GTCTAAG324000.011.7572171
TATACAG508700.011.4710245
TATATGT816450.011.3788244
TATAATA693600.011.352692
TCTATAC398300.011.34069253
TATTATA693750.011.3326382
Produced by FastQC (version 0.11.2)