FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_nw2-mss.3520000003eea0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_nw2-mss.3520000003eea0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846661
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT6000.0021683753215.37083220-224
TATACCG7500.004227755172.29668220-224
CGAGCGA7850.00433574482.30732220-224
GGGGGGA52106.647679E-874.408356220-224
CGACGGG8800.00577880773.421875220-224
TACCCCA48953.1841054E-452.79775220-224
GACCCCA64952.1390226E-549.73922220-224
CGACTAA15554.7303438E-541.550644220-224
TACGTTG16205.199352E-439.883488220-224
ACGAACG17653.8198777E-1136.60694220-224
GGGGGGG68900.036.46567215-219
TCTTTTA136901.3497938E-633.037163220-224
AGAACGA60750.001127488331.90679220-224
CGACGCA21855.2386895E-1029.570368220-224
CGGCCCC25350.001862173125.487673220-224
GACAGCG25451.4508923E-425.387526220-224
CACGCAA25805.16593E-1025.043121220-224
TCACGAA25850.002190652824.994678220-224
GCCGACA26956.354283E-623.97449220-224
CAAGAAA245901.9960862E-723.647875220-224
Produced by FastQC (version 0.11.2)