FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_nw1-mss.3520000003ee43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_nw1-mss.3520000003ee43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737748
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA11250.00309745960.058117220-224
TACCCCA36650.005628383355.305904220-224
GTGCGGG12602.7622096E-553.62332220-224
GCTATAC17001.0913936E-1139.744343220-224
TCTTTTA85608.328626E-539.46576220-224
CAACGAG17258.3345185E-739.168335220-224
ACCCAAA81200.00306943133.28344220-224
CAGACGA20502.579978E-532.958725220-224
TGTTTTA87250.00436404230.975533220-224
CCCCTAG22451.8299033E-830.095942220-224
GTAGTAG22903.1832315E-1029.504534220-224
CCTATAG23653.7536302E-728.568874220-224
GTACTTA23908.0035534E-1128.270035220-224
GGCCTAA23950.006922761428.211016220-224
GTTACAC24254.3051878E-627.862013220-224
CTCTTAA49702.0978769E-727.189287220-224
CTAATAG26551.7550934E-525.448357220-224
CTTAGGG26751.2812745E-425.258087220-224
TTAGCAC28050.001483779324.08748220-224
CCCGCCC28654.0593257E-423.58303220-224
Produced by FastQC (version 0.11.2)