FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_50330665-2-mss.3520000003efc8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_50330665-2-mss.3520000003efc8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466817
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGA4106.08695E-4329.01797220-224
TATCGGG4850.0010067286278.1389220-224
CCAACGC16655.8150406E-540.50972220-224
GCTTGGA33551.0843594E-440.20786220-224
GTCTAAC19551.5261321E-934.500603220-224
CTATTAA40954.0745363E-1032.94197220-224
CACTGCG20850.001349367832.349487220-224
AAAAGAT92200.005760471329.261902220-224
GGGTAAG23756.91216E-1128.399446220-224
TCTAGTA24151.9703075E-427.929062220-224
CCCTAAG24500.001515768227.530073220-224
GTCCTTA24959.093037E-727.03354220-224
CCCTAGG25406.0417457E-526.5546220-224
GTCTTAA27450.024.571468220-224
TACTAAG28901.0059452E-523.338644220-224
CAACCCG30104.480189E-722.408201220-224
CTCTATA30106.352457E-822.408201220-224
ATCTAAG30351.3351382E-922.22362220-224
GCGCCGG7600.002555899622.035751215-219
ATACCAA61900.00992771821.79279220-224
Produced by FastQC (version 0.11.2)