FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_50330665-1-mss.3520000003efaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_50330665-1-mss.3520000003efaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194700
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA23700.001152477682.557915220-224
CCCCACG37050.00677184352.81032220-224
CCCCCCG37550.00714049852.10713220-224
CCCCCCA100501.1098382E-745.427387220-224
ACTACGC16950.001487963738.47832220-224
AGAGCGT17800.001522321436.640873220-224
TAGACGG19205.0056534E-433.96914220-224
ACGTCAA19550.001341429433.360996220-224
ACGACAC43207.2759576E-1230.194794220-224
GGGGGGG70650.028.243904215-219
TGGCGCA24800.001807363826.298689220-224
CCGACAA26056.053697E-525.036757220-224
CAACCAA133800.001412337224.372477220-224
TCCAGTA54701.8189894E-1223.846708220-224
CCGACCA28801.595994E-622.646093220-224
CTAGTAG30652.5465852E-1121.279202220-224
CCCGGGG31154.708818E-720.937641220-224
ACCTAGA62353.6379788E-1220.920849220-224
CCCACGA31351.318409E-620.804068220-224
GCGGGGG31401.3592307E-620.770939220-224
Produced by FastQC (version 0.11.2)