FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_50110001-2-mss.3520000003ef78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_50110001-2-mss.3520000003ef78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358326
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA13454.2465035E-7200.15132220-224
GCGGCAT7600.0038897863177.10759220-224
ACTCCCG15852.0510821E-4127.38338220-224
GGCGGGA20055.225937E-4100.69957220-224
ACTACCG8455.559202E-579.64602220-224
GGACCGG10350.006220652765.02501220-224
CCGCCGA10353.9021843E-465.02501220-224
GTACGGA10359.931682E-1065.02501220-224
CTGCGCA11550.00999270958.26916220-224
CCCCCCA75506.482387E-753.484146220-224
ACCGGGG13600.003907060749.485943220-224
AGGGGGA55204.6965832E-448.768753220-224
ACGACGA16104.816957E-741.801792220-224
GCTAGTA18200.001932668836.978508220-224
AGACGAG22509.446694E-529.911507220-224
GGGTTAG26150.00346771825.736475220-224
GTAGTAG26351.5753354E-525.541134220-224
GGGGGGG62400.024.914082215-219
CGCTCCA27350.001076457924.60727220-224
TAAGGGC27354.1634466E-624.60727220-224
Produced by FastQC (version 0.11.2)