FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_41442-mss.3520000003ef35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_41442-mss.3520000003ef35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261478
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGT8950.006702367147.64691220-224
ACCCTCG9200.0072777937143.63477220-224
TTCGGGA10150.009762648130.19112220-224
GGGGGGA37450.006702244752.92816220-224
GTACGGA13304.9371796E-449.678192220-224
GACGCAA13451.380995E-749.124153220-224
CCGCCCC27201.0862459E-748.582344220-224
CGCAACA13753.0658604E-548.052357220-224
CCCACGA15550.004279927442.490025220-224
CGAACAA19456.932646E-533.970173220-224
ACACGAC20050.00217698632.953613220-224
GGAGAGA100700.00981599826.24508220-224
AGACAAA151158.59027E-526.227716220-224
CCGACGG6550.001041931125.70936215-219
GTACAAA52558.3491614E-1025.146332220-224
GTCTAAG26550.024.885872220-224
GCACTAT29401.3510142E-522.473467220-224
GGGGGGG57950.022.08476215-219
CTAAGAC31701.7117054E-720.8429220-224
GCCTAAA31851.8701394E-720.74474220-224
Produced by FastQC (version 0.11.2)