FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_40825-mss.3520000003ef28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_40825-mss.3520000003ef28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195637
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG2751.8991013E-4485.4328220-224
ACTTTCG9900.008789756134.84244220-224
CGACGAG10201.251186E-565.43825220-224
CTAGGGG26255.475158E-1050.854866220-224
GTCTATA28750.046.432705220-224
CCCATAC29956.4195844E-444.572292220-224
ACGACGA17501.0776239E-738.141148220-224
TTTTTTA195901.0913936E-1137.47918220-224
CCACGAA20350.002712316832.799515220-224
ACCCAAA89650.005295189629.781153220-224
TCCTTTA95550.00722949727.942232220-224
CACGACA23954.7568574E-727.869314220-224
TACACAG73150.027.374031220-224
AAAACGA52202.0679705E-525.573565220-224
AGGAGGG79708.309823E-525.124346220-224
GGTGTTC27950.001433354923.880861220-224
GCTTATA28003.978642E-523.838217220-224
GTCCATA28100.023.753386220-224
GGATAGC29755.887747E-422.43597220-224
TACCTAG29856.187338E-422.360807220-224
Produced by FastQC (version 0.11.2)