FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_35011-1-mss.3520000003ef5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_35011-1-mss.3520000003ef5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4586605
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGA5250.0013498131252.25595220-224
CGACGAG10900.008664065121.49943220-224
GACGAAC13900.00832768395.27653220-224
TATTCCG16801.2613054E-739.414997220-224
CGCACCC17206.870249E-538.498367220-224
CGAACAA21705.101722E-530.514833220-224
TATAGAA66700.029.782843220-224
ACGCGGG5704.6864487E-429.475636215-219
CGCCCCC24101.8189894E-1227.476011220-224
CCCCCCC100650.027.37588215-219
ACACAAG97550.00832172927.152102220-224
GGGGGGG59600.024.80701215-219
ATACTAG28109.434425E-723.564837220-224
GGACCAT28755.2750693E-1123.032066220-224
TACTATC28954.03828E-422.87295220-224
TTTTTTA218254.1430165E-521.238045220-224
CAAAAAG190854.115316E-420.81756220-224
GAGTTAC32002.992922E-420.692873220-224
CTTCTTA64404.1836756E-1120.564346220-224
GTCTACA33600.019.707499220-224
Produced by FastQC (version 0.11.2)