FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_35010-mss.3520000003ee86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_35010-mss.3520000003ee86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452362
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC13600.00415653448.586594220-224
CGACCAA14206.879578E-546.533638220-224
AGACGAA34651.8560004E-638.14012220-224
ACCAACG17658.355164E-837.437828220-224
CGACGAC19651.2460077E-933.627357220-224
CCCGGGG19950.003803375233.121685220-224
TGTATTG40202.6739144E-1032.874508220-224
CGCCGAA20601.5200349E-632.076584220-224
GCTATGC20900.009082139531.616152220-224
TTTCTTA109504.044482E-430.172497220-224
ACGCAAA22255.364245E-629.697874220-224
CGACAAA25803.376408E-825.611536220-224
CCCGCCC25903.4894497E-425.51265220-224
TTAGGGG26101.35965365E-525.317152220-224
TGTATAA52304.2473403E-925.268744220-224
CCTATAG26953.2726912E-624.51865220-224
GTAATAC27101.0550139E-924.382938220-224
GTTACAC27703.3910248E-523.854788220-224
TACACCA57600.022.943668220-224
GGAAGGT58852.904926E-922.456335220-224
Produced by FastQC (version 0.11.2)