FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_35005-1-mss.3520000003ef0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_35005-1-mss.3520000003ef0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646996
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGG7950.004291532171.3541220-224
AACATCG14850.00159399291.73502220-224
ACCCCCG17301.3916988E-578.743645220-224
CGGACAA10401.7466168E-465.493515220-224
ATAGGGT28302.8709887E-448.136574220-224
GTATTAA33500.040.664627220-224
CACGACA20608.908256E-533.064686220-224
TATAGGG20604.0199666E-1033.064686220-224
CCCCCGG21053.5882404E-432.357838220-224
ACGACAA23851.1185639E-628.559013220-224
CTTATAG24508.585266E-827.801327220-224
GCCTTAG24600.0094654227.688313220-224
CCCCTAA24858.5472493E-727.40976220-224
TCAGAAC51605.18412E-1026.400484220-224
CATAGGG26105.666379E-426.097033220-224
GTACTGA26456.384653E-1025.751701220-224
TACACTA30659.0059984E-822.222921220-224
TGTAGGG31604.0927262E-1021.554827220-224
GTATCAA32302.423385E-721.087694220-224
TCTTAAG32556.761355E-520.92573220-224
Produced by FastQC (version 0.11.2)