FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_34344-1-mss.3520000003eed6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_34344-1-mss.3520000003eed6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30178174
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCA480650.058.278606220-224
CCCCGGA151751.3587851E-939.555115220-224
GGGGGGA245100.038.095486220-224
GGGGCGA158102.0299922E-937.966404220-224
ACCGGGA123654.796275E-737.756588220-224
CGGGGGA163802.8703653E-936.64523220-224
CCCCGCA216953.45608E-1133.81597220-224
CGCCCCA242453.6379788E-1233.010178220-224
CCCCCCG285950.032.653275220-224
GGGGGGG395250.031.792263215-219
CCCGTTA84500.004006990731.571274220-224
CCCGGGA202102.2275344E-829.700586220-224
CCCCCGA196655.811944E-727.13219220-224
ACCCCCA464150.025.864433220-224
GCGGGGA180659.22947E-625.843357220-224
CCCCCCC898600.025.305904215-219
CCGGGGA159701.18762626E-425.05735220-224
ACGGGGA160651.2364353E-424.909176220-224
CCCCACG239053.1821928E-622.319788220-224
GCGCGGA183953.0951938E-421.754059220-224
Produced by FastQC (version 0.11.2)