FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_34311-1-mss.3520000003eebc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_34311-1-mss.3520000003eebc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42526565
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA393150.046.37448220-224
CCCCCCA740650.042.850746220-224
CCCCCCG384650.029.843983220-224
GGGGGGG606950.028.461685215-219
GCCGGGA239003.2268872E-928.253723220-224
CCCCCGA259652.2424138E-723.406033220-224
CCCCCCC1321750.022.474686215-219
CACCCGA241203.0917872E-622.396814220-224
CGGGGGA228205.110936E-520.71362220-224
CCCGGGA294807.6144715E-720.61525220-224
GGGGCGA200455.0869235E-420.212442220-224
CACCGGA206006.1146996E-419.667883220-224
GCCCCCA676900.018.954079220-224
GCGGGGA250301.0401281E-418.88473220-224
CCCTTTA488601.02591E-917.9665220-224
ACCCCCA723050.017.744299220-224
CCCCGCA306702.4631745E-517.613667220-224
GCACGGA231400.001335451317.509005220-224
TGGGCGA347653.6899928E-617.481304220-224
CCACCCG388805.5651617E-717.3679220-224
Produced by FastQC (version 0.11.2)