FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_34098-mss.3520000003efe2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_34098-mss.3520000003efe2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115422
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG11750.00229639114.68263220-224
AGCCGAA18750.00246216171.867775220-224
CGCACGC10000.002253529267.376045220-224
CCGCCGA20950.00334096864.32081220-224
ACGACGC11002.0741614E-561.250954220-224
TAACCCG12652.9186222E-553.261696220-224
TAGACGG14901.7025741E-945.218822220-224
TTCCGTA19355.5418996E-434.81966220-224
CCCCATA41003.6328329E-632.866364220-224
CACGACC21550.001589111431.264986220-224
GCCTTTA45154.2091415E-929.845425220-224
CCGACCA22652.7324533E-429.7466220-224
CGACCCA23253.6254478E-628.978943220-224
GTAAGGC25504.1777643E-426.421978220-224
GTTGTAC26851.3657531E-425.0935220-224
ACGTGTG27451.8945459E-424.545006220-224
GTAGGGG28954.001384E-423.273243220-224
TTTTTTA233602.3393204E-623.073988220-224
CTCGCGT7050.023.0552259
CTAGCCC29452.112345E-622.878115220-224
Produced by FastQC (version 0.11.2)