FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_30749-mss.3520000003ee6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_30749-mss.3520000003ee6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4489878
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCG15401.8627518E-4130.50766220-224
AACGACG10754.1673047E-962.319935220-224
GCCGACA15150.003147175344.220417220-224
CCCCCCA62508.8607735E-442.87612220-224
CGCACAA16852.320791E-439.759014220-224
ACCCTAA36751.5585645E-536.459286220-224
GACGAAC18500.036.212936220-224
CCGCCGA19252.9338746E-534.802044220-224
TGTATGG40054.020767E-733.45515220-224
TGGTATA42105.9790545E-631.826096220-224
CGAACAG21951.959887E-430.521156220-224
TTAGTAC24400.00145518127.456532220-224
GGGGGGG51050.027.15161215-219
GTTTAGG28201.0025233E-623.756714220-224
CCCTAAG29301.9331874E-622.864824220-224
CTTATAG29851.7176E-522.44353220-224
TTTTTTA188203.4592062E-421.358322220-224
TACTAAG32008.632815E-620.935606220-224
GTAATAG32651.9498275E-620.518816220-224
TTAGCAC32702.003564E-620.48744220-224
Produced by FastQC (version 0.11.2)