FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_30521-mss.3520000003f00a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_30521-mss.3520000003f00a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461033
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC13003.4653021E-751.06319220-224
CCCCCCA78759.53838E-750.576878220-224
TCATCCG18250.002003402236.37378220-224
ACAGCGC18400.00219729136.077255220-224
CGACCAA18550.00506493335.785526220-224
GTGCTAC23852.4254055E-527.833185220-224
CCAAAAA144806.2169915E-527.506416220-224
ACGAAAA49601.1007711E-426.766994220-224
GTCCTAG27600.024.051502220-224
CTAGGGT27751.6934791E-923.921494220-224
CCTATAC28800.023.049358220-224
GGGGGGG61550.022.851389215-219
CCCCCCG29558.7071385E-822.464348220-224
GTGTGGA59400.022.350893220-224
GGCTAGG31151.9896723E-421.31048220-224
CACAAAA221004.4735127E-521.02602220-224
CATAAAA159200.003488893920.848663220-224
ACACTAA64750.002321792320.504139220-224
GTACAAC32806.8012014E-920.238459220-224
CCAACAA98702.7467663E-420.176945220-224
Produced by FastQC (version 0.11.2)