FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_30423-1-mss.3520000003f04e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_30423-1-mss.3520000003f04e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5791481
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACG8250.0053065633159.67345220-224
CGTGCTA8800.0064361803149.69386220-224
CACCGGA16752.7879077E-4117.9677220-224
CGGCGGA8905.2406674E-474.00596220-224
GGGGGGA48853.5233898E-667.41586220-224
CCCCCCG32650.003948162360.519413220-224
ACCGATA11000.00884053659.877544220-224
CGTACCC14600.001835702145.113216220-224
ACCGAAC18456.4704387E-435.69935220-224
ACTCACG20855.07042E-431.59007220-224
CTATTAG42200.031.21578220-224
CGACACA45603.092282E-1128.888287220-224
GACCGCG5805.319299E-428.845215215-219
AGACGAA47255.1832103E-827.879492220-224
GCAACGA24152.2993496E-427.273415220-224
CCCACAA97050.00834199227.146954220-224
CGACCAA24806.767059E-726.558588220-224
GCGGGGG25108.735871E-626.241156220-224
CCGACCA25554.2486863E-725.778982220-224
TACACTA51451.2732926E-1125.603613220-224
Produced by FastQC (version 0.11.2)