FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_30245-mss.3520000003ee50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_30245-mss.3520000003ee50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5176401
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGA6858.165804E-6285.9794220-224
ACCCCCG19605.388891E-499.946884220-224
CACCCCG24900.001394643178.67305220-224
CACGAGA32950.004234689859.452477220-224
CGACTAA11309.2979876E-457.7864220-224
AGCGAAA26750.002775272648.821407220-224
CCCGTGC15854.064155E-441.197872220-224
AACAGGA111851.1949989E-535.028324220-224
CCGACAA18859.193448E-434.641186220-224
GTCTCGC19401.2652163E-733.659092220-224
AACACGA61150.00824552732.03531220-224
GCCGACA21301.8144761E-530.656637220-224
CAAGGGT43403.8198777E-1130.091536220-224
AGCGACA22702.9533057E-428.765917220-224
GTTAGAC22902.220404E-728.514688220-224
CACGCAA22956.661957E-428.452564220-224
ACGAGCA23204.0241945E-428.145964220-224
CCATAGA47754.4019544E-1027.350214220-224
CAACGAC25252.3878292E-625.860844220-224
CTTATAA77000.025.441027220-224
Produced by FastQC (version 0.11.2)