FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n02_30230-1-mss.3520000003f024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n02_30230-1-mss.3520000003f024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554966
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGA5650.0016326505236.7226220-224
CCGCGGA5800.0017658712230.60046220-224
CGACCAA16907.2582498E-639.570496220-224
TAGGGGA35402.1540836E-737.781998220-224
GTATAGA36950.036.197094220-224
CCCAACG18651.2348301E-435.85744220-224
TTAACCC40552.1809683E-932.983543220-224
CACGCAA21050.003768011531.769182220-224
ATTTCTA85400.004139295331.322779220-224
AGACGCA21500.001629144531.10425220-224
CACCAAA108703.6121125E-430.760868220-224
GTGCTAC22803.092282E-1129.33076220-224
ATACGAT23554.361209E-828.396658220-224
TAGTAGT25303.7456225E-426.432463220-224
CCCTATA25450.002469045326.276674220-224
CCCCCCG25454.7769066E-526.276674220-224
GTACTTG26250.003664499125.475859220-224
GTACTAA26353.0916817E-725.379177220-224
GCTCATA26908.5505913E-424.860273220-224
TAAGGGG27650.00123422524.185944220-224
Produced by FastQC (version 0.11.2)