FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_nw2-mss.3510000003eea3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_nw2-mss.3510000003eea3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846661
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA2452.3249068E-433.22119245
TCGTATT24800.021.004366245
GTACGGA26600.019.7120721
GGATCGT22200.019.2147738
ACTGATC91400.018.0014638
GTGTATA112900.017.821141
GTATATA120900.017.751371
TATACTG67150.017.6109315
GATCGTT25750.017.512369
GTATAAG56400.017.0802171
GTATAGG35000.017.0715711
AGGATCG26800.016.8262427
GTATAGA57500.016.7534681
GTCTAAG47750.016.5991341
GTGTAGG45600.016.3121221
GTGTAAG72950.016.046911
GTATTAG55650.015.7765761
GTATTAA57200.015.7754291
CTCCGAC21800.015.681012245
GTACCGG7051.1148499E-515.5667811
Produced by FastQC (version 0.11.2)