FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_nw1-mss.3510000003ee46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_nw1-mss.3510000003ee46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737748
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG3150.00542147319.3550915
TAGGGCG4009.976538E-418.2905625
GTGTATA59050.018.1747441
GTATAGA34150.017.1417871
TATACTG41350.016.8087395
GTATAGG21300.016.6044271
GTATTAG34800.016.4711591
GTATACG5954.5667555E-516.3949013
GTGTAGA41800.016.3386921
GTATATA63300.015.99115851
ACTGATC57150.015.78317458
ATACTGT56550.014.6626386
GTGTTAG42450.014.3647421
TCCGTAC10251.2621058E-714.275563
GTCCTAG29150.014.224791
TAATACT43550.013.4396774
AGTACCG5500.00832946313.3022275
GTGTTAA42250.013.2781221
GTATAAT40600.013.2169781
GTGTAGG31450.013.1845041
Produced by FastQC (version 0.11.2)