FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_50330665-2-mss.3510000003efcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_50330665-2-mss.3510000003efcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466817
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG22850.018.6790851
GTATATA61650.017.209171
GTATAGC18550.016.4350051
TCGTATT10101.3115023E-616.005177245
GTATAGA27800.015.7917941
ACTGATC58250.015.69395358
ACCGATC4700.002942112715.5603888
GTGTATA55500.015.3807961
TATATAG35050.014.6103232
GTATAAT37200.013.7682931
TACACTG55850.013.53527455
ACACGGT17501.8189894E-1213.2377476
TAGGACG6450.001558956113.23234754
GTATTAG31450.013.1835381
ATTATAC42950.013.0584973
GTACTGA29000.013.0335376
TATTATA55750.012.9034462
GTACTAC15357.021299E-1012.7111651
CTTACAC46100.012.695183
CTGTCGC19351.8189894E-1212.5984189
Produced by FastQC (version 0.11.2)