FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_50330665-1-mss.3510000003efa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_50330665-1-mss.3510000003efa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194700
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG7754.0363375E-918.877435
GTATACG8455.7843863E-1018.7612691
TATACTG58650.018.7084525
GTATATA90300.017.6912751
TCCGTAC14050.016.4869613
GTGTAGA61450.015.280741
GTATAGG35350.015.1788481
TATACAG71900.015.0912255
GTATTAG54150.015.0887031
CCGTACC13757.2759576E-1215.0733434
GTATAAG48850.014.97829151
ACTGATC81850.014.8908148
GTATAAT59150.014.8440811
GTGTATA77700.014.4391751
GTATAGA52100.014.0439441
GTCTAAG49550.014.0283561
GTGTAAG66650.013.9055781
TATATAC99450.013.8527813
GTATATG73300.013.8086021
GTCCTAG50450.013.7780981
Produced by FastQC (version 0.11.2)