FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_50110001-2-mss.3510000003ef7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_50110001-2-mss.3510000003ef7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358326
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA67200.017.4217931
GTATATA78150.017.0093771
GTATAGG25900.016.9509331
GTATCGT5904.2531683E-516.5317636
GTATTAG42250.016.452771
CCTATAC27050.016.2261873
TATACTG45650.016.0247655
GTATAGA38100.016.004271
GTACGGT5404.061866E-415.8046723
CGTACCA13405.456968E-1215.4676865
CTATACG6408.525354E-515.2402214
CCGTACC11351.7898856E-915.0388074
CTGTCGC21750.014.5702089
CCACGAA21400.014.390608245
TCTGTCG26300.014.3667188
TTAGCGC5100.005056311414.3437363
TAATCCG6009.0342324E-414.2242065
TCCGTAC12355.8826117E-913.821093
TATATCG6250.001229849913.6552375
TAGGACC25950.013.6251684
Produced by FastQC (version 0.11.2)