FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_41442-mss.3510000003ef38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_41442-mss.3510000003ef38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4261478
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGC3300.007091934318.4755041
GTATAGG23650.018.0458431
GTCGTAG3450.00916304517.6722241
GTGTAGG31550.017.0056651
GTGTATA69200.016.740091
GTATATA77350.016.7103611
GTGTAGA43100.016.4093361
GTATAGA36750.016.2584461
GTATAGC21800.016.2211551
TATACTG45750.015.9890995
ACTGATC62600.015.9660078
GTATTAG38700.015.1241351
TATACAG49150.014.8830365
TCTATAC38700.014.4914113
ATACTGT66350.013.9648576
ATAGGAC30650.013.9219923
GGATCGT15103.8198777E-1113.7223598
TAGGACC26000.013.5984214
GTCTAAG32300.013.5906511
GTATTGG35250.013.491051
Produced by FastQC (version 0.11.2)