FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_40825-mss.3510000003ef2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_40825-mss.3510000003ef2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195637
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA78400.020.9997431
GTGTATA69500.019.4775541
GTATAGG25000.017.5613841
GTATTAA46650.016.2082561
CTGTCGC22050.015.4782949
GGATCGT15800.015.4294968
ACTGATC61350.015.1000328
TAGACTG49950.014.1582995
GATCGTT16600.013.9514459
TCGTATT14051.8655373E-713.825748245
GTATAAG39800.013.7887761
TATACAG55500.013.6212625
ACGGTTC8104.1479645E-513.543678
GTACTAG19850.013.5163181
GTATAAT45350.013.4458721
TATAGTG36600.013.325935
GTGCGCG5500.00832202713.3040791
AGGATCG19250.013.29742057
GTGTAGG32250.013.2353241
CCTATAC28600.013.2164333
Produced by FastQC (version 0.11.2)