FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_35011-1-mss.3510000003ef52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_35011-1-mss.3510000003ef52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4586605
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC13550.026.340984245
TCGTATT15950.018.308823245
TATACTG53450.015.2833485
GTCCTAT30000.015.0401591
TCGTAAC5756.54371E-414.8429982
ACTGATC68850.014.6936018
CCTACAC49000.014.6807213
GTATATA85900.014.6223111
TATAGTG40600.014.4147345
GTCTAAG40400.014.186931
GTCCTAG36450.014.0515331
GTATTAG46150.014.0047741
GTGCTAG43150.013.5653931
TACACTG72000.013.5471815
CTTACAC62400.013.482053
GTGTAGA49050.013.4253841
TGCTAGA66850.013.3141322
GCGTATA5550.00883816113.1834851
GTATTAT58450.013.1440131
GCGCTAT6500.001650343613.132781
Produced by FastQC (version 0.11.2)