FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_35010-mss.3510000003ee89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_35010-mss.3510000003ee89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452362
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA75600.019.6813161
GTGTATA69650.017.6854611
GTATAGG23350.017.2362651
TATACTG46150.016.9091575
ACTGATC62050.016.3038048
GTATTAT46100.015.8732711
GTATAGA38900.015.0490041
CCGTGCA13256.91216E-1114.718279
GTGTTAG46600.014.6560941
TATATGT90400.014.5669624
GTATACG6701.2561843E-414.56234551
TAGCGGT5958.475166E-414.3448084
GTATTAG38750.014.16305351
GTGTAAG47100.013.9826341
GTATATG65450.013.97551251
GTATTAA44550.013.9617091
TAGGACC23700.013.8908594
TTATACT52900.013.8295874
GTCTTAG33750.013.7317521
TAATAGT41100.013.6467644
Produced by FastQC (version 0.11.2)