FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_35005-1-mss.3510000003ef01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_35005-1-mss.3510000003ef01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646996
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG35250.017.2995681
ACTGATC60600.016.2931278
GTATATA63200.016.210131
AGGTCGT6459.095838E-515.1247816
GTATTAC24650.014.3484641
GGGCCGC10201.1880729E-714.34636
TATACTG36550.014.34635
CTGATCA66600.014.0927799
GTGTATA59600.013.9151211
GTATTAT42200.013.8724491
CTCGCGT7051.9448849E-413.8318749
CATACCG5300.006516984713.8049314
GTATAGA33800.013.3508631
GTATACA40350.013.2994441
CCTATAC25850.013.2085853
GTTAGCC33250.013.202913
TACACTG53750.013.158565
GTATTAA40900.013.12061
ATACTGT55050.013.0693356
GTATAGG23650.012.8923841
Produced by FastQC (version 0.11.2)