FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_34344-1-mss.3510000003eed9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_34344-1-mss.3510000003eed9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30178174
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA406100.018.3484461
TATACTG292550.015.7124975
GTATAAG260450.014.9367931
GTATAGA255300.014.7604211
GTATAGG187000.014.543011
GTGTATA358400.014.49548151
GTATTAG293000.014.4844721
GTATTAT362200.014.24239351
TACACTG374550.013.8351255
GTCCTAG185350.013.5539451
GTATAAT297000.013.3860441
TATATAC421850.013.382183
GTGTAGG282650.013.245871
GTATATG370750.012.828461
TATAATA440700.012.505452
GTATTAA341300.012.3274681
GTGTAAG293000.012.3201261
TATACAG340650.012.312725
TATTATA441150.012.133392
GTGTAGA330950.012.0865551
Produced by FastQC (version 0.11.2)