FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_34311-1-mss.3510000003eebf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_34311-1-mss.3510000003eebf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42526565
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA534950.017.2803421
GTATAGA338750.016.6325571
GTGTATA497150.016.067571
TATACTG370950.015.6458825
GTATTAG373600.015.6359761
GTATAGG250250.015.5945281
ACTGATC589050.014.9815248
GTATAAG346450.014.5028371
GTGTAGG375500.014.1927921
GTATTAT424050.013.7757361
GTACTAG195900.013.7579641
TACACTG535000.013.6743275
TATACAG460250.013.3519665
GTGTAAG417250.013.12341
GTCCTAG289500.012.8483571
GTATTAA413150.012.8108611
GTATAGC234750.012.7798531
GTATTAC317300.012.5682281
TCTATAC344950.012.4421383
GTATATG485650.012.3799781
Produced by FastQC (version 0.11.2)