FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_34098-mss.3510000003efe5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_34098-mss.3510000003efe5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115422
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37250.021.9341161
CGAGCGA4450.007640085618.238497245
GTCCTAC28650.017.8770681
TGCTAGA73700.017.039152
TATACTG56200.016.7044665
GCTATAC26050.016.3809283
CCTATAC33600.016.3286933
GCTAGAC52700.016.194433
CTATACT47450.015.6737114
GTACTCG13605.456968E-1215.2401146
ATGCTAG54700.015.1597911
GTATAGG29100.015.0862451
GTATAAG47000.015.0487981
CTACACT69350.014.9434424
GTGCTAG46550.014.9323031
TACACTG77500.014.7878275
TCTATAC47300.014.6923723
GTGTATA84950.014.3551671
TAGATCG5958.48071E-414.34363655
ACTCGCG9407.4373384E-714.2632978
Produced by FastQC (version 0.11.2)