FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30749-mss.3510000003ee6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30749-mss.3510000003ee6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4489878
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT29700.018.0656851
GTCTTAG36750.017.5863931
GTATATA91550.017.1826271
CCGTACC13500.017.158774
GTCCTAC23100.016.3646971
GTGTATA77500.016.0493241
TATACTG49100.015.8914965
GTGTAGG33150.015.449841
GTACTAG21500.015.313821
GTCCTAG30350.015.2680351
GTCTTAC33750.015.1751761
CTATACT44600.015.0346784
CCTATAC32500.015.00523953
TCTATAC45700.014.939573
CGCTAGA7451.8822624E-514.7282972
GTGTAGA45850.014.6278871
ACTGATC69200.014.6193168
GTATTAA46900.014.5604031
GTGCTAA37950.014.4596891
GCTAGAC45000.014.359183
Produced by FastQC (version 0.11.2)