FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30521-mss.3510000003f00d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30521-mss.3510000003f00d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461033
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC12350.017.7708873
CCGTACC12400.017.6992284
GTGTATA67100.017.628331
CGTACCA13750.015.9614875
TATACTG46700.015.9263585
GTATATA70600.015.8907761
GTATAGA35000.015.3301561
GTATTAT46250.015.2924891
GTATTAG42650.015.1537051
ACTGATC76750.014.6108898
GTGTAGG36150.014.1678181
TCGTATT13751.4231955E-714.135793245
GTATAGG26800.014.1055121
GTACCAT52900.014.0597526
GTCCTAA35050.013.9166261
TAATGCG6250.001229381513.6559384
GTATTAC29900.013.458751
GAGTACG7302.603884E-413.3637721
GTGCTAG41400.013.2548281
GTATAAT41500.013.2228891
Produced by FastQC (version 0.11.2)