FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30484-1-mss.3510000003f05e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30484-1-mss.3510000003f05e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6588
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT70.10625379477838494No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT100.0014633377243.854978
TATACTG150.004923952162.569985
AACAGTG150.004923952162.569987
TATAGCA150.004923952162.569984
CCCCCCC150.009222357548.770992180-184
Produced by FastQC (version 0.11.2)