FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30423-1-mss.3510000003f041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30423-1-mss.3510000003f041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5791481
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA50500.019.0771961
GTATATA95300.018.6826631
GTATAGA47900.017.821381
GTATAGG31800.017.6404441
GTGTATA83300.017.5677051
GTATTAG52650.017.140051
GTACGCC5700.001557504317.11498245
TATACTG55400.016.94615
TATACGG7257.826329E-716.8170855
GTATTAT55200.016.79011
GTATAAG47450.016.4483591
CTATACT50100.016.0618294
GTGTAGG42950.015.9002391
CTGTCGC24950.015.143769
GTACTAA33800.014.4318521
TCTATAC51050.014.0911043
GATCGTT19900.014.086969
GTCTTAG45050.014.07626151
GTATAAA89450.013.9058851
TAGGGCG6150.001088763713.8775154
Produced by FastQC (version 0.11.2)