FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30245-mss.3510000003ee53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30245-mss.3510000003ee53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5176401
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATT16500.018.694939245
GTATATA93850.018.0617641
GTATAGA44800.017.1491341
GTGTATA81850.015.9420721
CTAGTAC27300.015.6321213
ACTGATC80600.015.12268
TCCGTAC12203.092282E-1014.9914613
GTCCTAA41650.014.6397831
GTATTAG49150.014.638891
CCGTACC13357.6397555E-1114.61339954
TCTATAC48100.014.449153
GTCCTAC30700.014.3002551
GTATAGC25100.014.0897721
GTACGGA13058.458301E-1014.0171731
TATACAG66350.013.9664225
GTATTAA54750.013.8097951
CTATACT47700.013.8034574
GGGTACG6200.00115556713.768481
GTCCTAT32900.013.7146731
CGCTAGA8956.98827E-613.6235242
Produced by FastQC (version 0.11.2)