FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l01n01_30230-1-mss.3510000003f027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l01n01_30230-1-mss.3510000003f027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554966
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA38400.018.0998691
TATACTG45050.017.8609525
GTATTAG36900.017.8442881
ACGGACC22950.017.675117245
TACACTG62450.017.569755
TCTATAC39350.016.110693
ACTGATC76100.016.0164388
TATACAG49300.015.82663255
GTATAGG27300.015.6328151
GTGTATA68850.015.2309261
GTATATA69850.015.1874411
GTATTAT45300.015.073761
GTACGGA18750.014.9574771
GTGTAGG37050.014.8100351
GTATTAA44150.014.6378391
TATATAC75900.014.2956443
CCGTACC13701.1641532E-1014.23819354
CTGATCA87700.014.0369389
GTATATG59500.013.7306021
GTATGCG5450.00784237213.4241421
Produced by FastQC (version 0.11.2)