FastQCFastQC Report
Sat 9 Jul 2022
HCJCVDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJCVDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028814397
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGG267278621.3174128712573405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGGAC97814720.48212749349885453Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGGACTAC61639440.30382000488140265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTCGCAGTGACCAGGC56764500.2797914884867608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGGA46902460.23118162050384936Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGGACT41550730.2048030123477086Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGCCGGCGACGCATCATTC30213550.14892219832763734Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGACGGGACTA27887010.13745471266980563Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC26901400.13259665368985452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCGGCGACGCATCATTCA22040570.10863768530325547Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)