FastQCFastQC Report
Fri 8 Jul 2022
HCJCVDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJCVDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028814397
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTCGTA1109235445.4674071794848365No Hit
CACCTGCC1013307254.994578368028015No Hit
TCACTCGC969943764.780840285016964No Hit
CCAGGAGC946317784.664388134268548No Hit
CGGGCTTA928884084.578457651786863No Hit
TGATGCAT815949994.02180697853161No Hit
GATCTCAG813150334.008007490494952No Hit
GCTACTGA810046153.992707027305268No Hit
AGTCCCAC789269953.8903014054271816No Hit
CTATAGTG786129363.8748214778170267Clontech Universal Primer Mix Short (100% over 8bp)
TTCATCGG784990753.8692092838101053No Hit
AGCATGGT775604973.822946895225527No Hit
ATGGAATG768379653.787333386120485No Hit
TACGTTCA759865073.7453651311012455No Hit
GCTTGGCT755401763.723365533668381No Hit
GTGACTTG737901123.6371051047899283No Hit
GCGAGAGT734385533.6197768070156298No Hit
ATGAATCT712596773.512380289955129No Hit
CAAGCTCC710028643.499722010302749No Hit
CATGAACA695212213.4266920178997524No Hit
AAACAAAC682924063.366123885013026No Hit
AGCTGGAT637895353.1441779540960146No Hit
GGGGGGGG626222053.086640408930418No Hit
GTTCACTG621495723.063344389309359No Hit
TCGTGAAA601969382.967099311253557No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)