FastQCFastQC Report
Wed 23 Nov 2022
HCJ5FBGXN_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJ5FBGXN_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372260308
Sequences flagged as poor quality0
Sequence length6
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAA69344971.8628085914547732No Hit
TAAAAA33127430.8898996021891219No Hit
CAAAAA22836010.6134419788853772No Hit
AAAATA19455540.522632673478581No Hit
AATAAA19355020.5199324124558561No Hit
AAAAAT17517420.4705691051005094No Hit
ATAAAA17432040.4682755487324209No Hit
AAATAA17060030.45828227273695804No Hit
GAAAAA16058200.43137019056031084No Hit
TTAAAA12807670.3440514533717089No Hit
AAAACA11958370.32123677284444735No Hit
ACAAAA11261080.302505525246597No Hit
AACAAA11160660.2998079505161748No Hit
TATAAA10982960.2950344090941869No Hit
TAAATA10732780.2883138430111652No Hit
TAAAAT10269170.27585992326638276No Hit
AAAAAC9993250.26844790554463305No Hit
TAATAA9454310.2539704018081885No Hit
AAACAA9348100.251117290753437No Hit
AAAATT8479100.2277734106425335No Hit
AATTAA8323150.2235841377963938No Hit
AATAAT7978930.21433738243186537No Hit
AAATTA7927380.2129525987497974No Hit
CTAAAA7922040.21280915074083054No Hit
AATATA7840900.21062949316637863No Hit
AAATAT7681790.206355333483472No Hit
TCAAAA7478900.20090511503042113No Hit
CATAAA7387860.19845951451799693No Hit
GTAAAA7385540.1983971925365731No Hit
ATTAAA7233140.19430328306718103No Hit
ATAATA6994310.18788761116052158No Hit
CAAATA6962650.18703713101747071No Hit
CAAAAT6672980.1792557481040928No Hit
CCAAAA6554850.1760824310068534No Hit
ATATAA6542240.17574368954747654No Hit
ATAAAT6400110.171925662297577No Hit
TAAACA6366940.1710346191407546No Hit
TTTTTT6361100.17087773967027395No Hit
CAATAA6238510.16758461393633187No Hit
TACAAA6028000.1619297000098114No Hit
TTTAAA5852460.15721418250156285No Hit
TTAATA5811970.1561265027481791No Hit
AATTAT5782700.15534022499116398No Hit
AAATTT5710300.15339534936397248No Hit
AATTTT5629890.15123530172333066No Hit
TAAATT5623650.151067677083639No Hit
TAAAAC5571300.149661403063149No Hit
GATAAA5472410.14700492860495887No Hit
GGGGGG5428160.14581624426099168No Hit
TTAAAT5417240.1455229011415313No Hit
TATAAT5341480.1434877660929674No Hit
AATTTA5229830.14048852073694626No Hit
GCAAAA5215300.14009820246535656No Hit
TTATAA5193750.1395193064741138No Hit
TATTAA5185150.13928828533607723No Hit
ATTTTT5176520.13905645831034985No Hit
AATATT5175430.13902717772424988No Hit
TAATTA5094320.1368483260374888No Hit
TATATA5064380.13604405012204523No Hit
TAACAA5034510.13524165461121362No Hit
TAATAT5019790.1348462323842487No Hit
GAAATA4978420.13373491325860076No Hit
GAAAAT4935640.132585717411484No Hit
AAAAGA4771940.12818825691188113No Hit
TATTTT4603420.12366131712328568No Hit
CAAACA4462840.11988492740407876No Hit
AAAACT4455550.11968909669520823No Hit
GAATAA4435730.11915667356080305No Hit
ATTTAA4432290.11906426510558843No Hit
AAGAAA4298960.11548263157833093No Hit
CACAAA4273610.11480165647958364No Hit
TAATTT4238200.1138504403751796No Hit
ACTAAA4222920.11343997491131931No Hit
AATACA4183380.11237781493481169No Hit
TATTAT4176690.11219810197975767No Hit
ATAATT4168880.11198830255091284No Hit
TTATTT4167590.11195364938020737No Hit
AAAATC4162150.11180751507893771No Hit
CAAAAC4125300.1108176163653741No Hit
TTTTAT4124990.11080928885923558No Hit
ACAATA4121190.11070720975173104No Hit
ATTAAT4079610.10959024941224732No Hit
AACAAT4069320.10931382993429425No Hit
ATATTA4067860.10927461006667409No Hit
AACTAA4056120.10895923935033117No Hit
ATATAT4055020.10892969013500092No Hit
AAATCA4042120.10858315842794608No Hit
TTAATT3988460.10714169397829006No Hit
ATTTAT3977800.10685533521881682No Hit
ATTATA3972590.10671537938984352No Hit
AACATA3958670.10634144750130063No Hit
AATAAC3946660.10601882379574026No Hit
ATATTT3921490.10534268402313791No Hit
TATATT3885920.1043871698510495No Hit
TTTTAA3883040.10430980463273028No Hit
ATAACA3881080.10425715330359636No Hit
AGAAAA3867930.10390390586578464No Hit
TTTAAT3826700.10279634754936054No Hit
AAACTA3790240.10181692537577765No Hit
TGGGGG3786310.10171135408827954No Hit
AATCAA3781940.10159396311464934No Hit
TTTATT3778880.10151176257018517No Hit
AAAACC3751370.10077276355769844No Hit
TTATAT3735990.10035961180153538No Hit
TTATTA3726000.1000912512004906No Hit
TATTTA3725880.1000880276497273No Hit
TTTTTA3724430.10004907641133741No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)