FastQCFastQC Report
Wed 23 Nov 2022
HCJ57BGXN_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJ57BGXN_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389501610
Sequences flagged as poor quality0
Sequence length6
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAA62026051.5924465626727446No Hit
TAAAAA25659130.6587682654251417No Hit
AAAATA21663100.556174851241308No Hit
AATAAA18692280.47990250926048805No Hit
AAAAAT18433730.47326453926596096No Hit
ATAAAA16302760.4185543674646171No Hit
AAATAA15870940.4074678921096116No Hit
CAAAAA15649130.40177317880662927No Hit
AAAACA14441750.3707751041131769No Hit
TTAAAA12180360.3127165507736926No Hit
AAAATT12034590.3089740758709572No Hit
AAAAAC11145760.28615440126165337No Hit
TATAAA11072900.28428380565615635No Hit
AACAAA10364490.2660962043263441No Hit
TAAATA10094120.25915476960416156No Hit
ACAAAA9929440.2549268024848472No Hit
GAAAAA9614600.24684365232790695No Hit
AAACAA9343960.23989528567032112No Hit
ATTAAA9137290.23458927422662No Hit
TAAAAT8908090.22870483128426605No Hit
TTTAAA8851470.22725117875635995No Hit
AAATTA8679660.2228401571947289No Hit
TAATAA8482200.21777060176978472No Hit
AAATAT7842800.20135475178138543No Hit
AATATA7720100.19820457224810958No Hit
AAATTT7590550.1948785269462686No Hit
AATTAA7454430.19138380454961407No Hit
AATAAT7371810.18926263231620533No Hit
AAAACC6626060.17011636999395202No Hit
AAAACT6518640.16735848665683306No Hit
CTAAAA6512130.16719134999210913No Hit
AAAAGA6497080.16680495877796242No Hit
TCAAAA6420170.164830384141416No Hit
TAAACA6419650.16481703374730594No Hit
TAAATT6406090.1644688965470515No Hit
ATAATA6358380.1632439978874542No Hit
ATAAAT6232760.1600188507564834No Hit
TTTTTT6156250.15805454565386778No Hit
CATAAA6113240.15695031401795748No Hit
AAAATC6088580.15631719725112306No Hit
CAAATA6070570.15585481148588834No Hit
GTAAAA5969330.1532555924480004No Hit
ATATAA5911130.1517613752610676No Hit
NNNNNN5846330.15009771076427644No Hit
TTTTAA5720200.14685946997754387No Hit
CCAAAA5618370.144245103377108No Hit
TACAAA5477970.14064049696739378No Hit
TATTAA5427390.1393419144018429No Hit
AATATT5379640.1381159887888525No Hit
CAAAAT5324800.13670803568693848No Hit
TTATAA5224520.1341334635304845No Hit
TTAAAT5136490.13187339585066157No Hit
TAAAAC5089230.13066005041673642No Hit
TAATTA5068920.13013861483139955No Hit
AATTTT5034610.12925774555848432No Hit
TTAATA5024250.12899176462967638No Hit
AAATCA5017670.12882283079651455No Hit
TATATA4997320.1283003682577846No Hit
TATAAT4925170.1264480010750148No Hit
ATTTAA4924550.12643208329742206No Hit
TAATTT4921010.12634119792213439No Hit
CAATAA4837480.12419666249903305No Hit
TTTTAT4820990.12377330096273542No Hit
AATACA4813130.12357150462099502No Hit
GGGGGG4772270.1225224717299628No Hit
TAATAT4755500.1220919215199136No Hit
ACTAAA4753510.12204083058860783No Hit
TTTAAT4725270.12131580149309266No Hit
AAACTA4630330.11887832761461499No Hit
AATTAT4618050.11856305292293914No Hit
AATTTA4617210.11854148690168445No Hit
AAAAGT4568560.11729245483734971No Hit
TAACAA4531160.11633225341481901No Hit
GTTAAA4530050.116303755458161No Hit
ATAATT4433690.11382982473422895No Hit
GATAAA4385940.11260389912123854No Hit
TATTTT4361900.1119867001319969No Hit
CTTAAA4357810.11188169414755435No Hit
ATCAAA4328960.11114100401279471No Hit
ATTAAT4307460.11058901656401368No Hit
GCAAAA4233640.10869377407708275No Hit
TTTATA4221830.1083905660877756No Hit
ATTTTT4202710.1078996823658829No Hit
AAAAAG4196130.10773074853272109No Hit
AAACCA4194360.10768530584507724No Hit
AACATA4192230.1076306205768957No Hit
TTTTTA4127410.10596644260340798No Hit
AAGAAA4098680.10522883332882758No Hit
TTTATT4098450.10522292834681736No Hit
ATTATA4075410.10463140319240272No Hit
TTATTT4064910.10436182792671897No Hit
AAATAC4040430.10373333245015343No Hit
AAACAT4028930.10343808334964263No Hit
CACAAA4014160.1030588808092475No Hit
AATAAC3990170.10244296551174718No Hit
TCTAAA3978890.10215336465489834No Hit
CAAACA3971340.10195952720195431No Hit
ATAACA3960560.101682763262519No Hit
TTAATT3932180.10095413983012805No Hit
TATATT3913890.10048456539114177No Hit
AACTAA3898370.10008610747462635No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)